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No conjugate is needed for fluorescent blot detection. Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN 3, 0.01% bromophenol blue.įluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. Standard plugs are offered for 2-D electrophoresis applications: Precision Plus Protein Unstained Standard Plugs, Strep-tagged, recombinant, pkg of 24 For blot development, Precision Protein StrepTactin-HRP Conjugate or StrepTactin-AP Conjugate should also be ordered. Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range.Molecular weight sizing of proteins after blot development.Monitoring blot transfer efficiency with easy orientation via multicolor standards.Use improved Precision Plus Protein Dual Color Standards for molecular weight estimation on SDS-PAGE gels and western blots. (Note: Actual weights may vary lot-specific MWs are included with each vial. These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. Precision Plus Protein Unstained Recombinant Protein Standards are Strep -tagged, enabling immunodetection and molecular weight determination on western blots. Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. LC-MS innovation: Improve analytical accuracy Note: For Precision Plus Protein Dual Xtra Prestained. These standards can be detected by fluorescence when excited at red and green wavelengths. Which microbioreactor modules are best for you Precision Plus Protein Dual Xtra Standards are a mixture of 12 recombinant proteins (2250 kD), including nine blue-stained bands and three pink reference bands (2, 25, and 75 kD). Which solid support is right for your oligo synthesis? Cell & Gene Therapy Accelerating ScienceĬell & Gene Therapy Accelerating Science Feature.NEW: Ensuring Food Quality, Safety & Authenticity.SLAS2024 International Conference and Exhibition.Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN 3, 0.01% bromophenol blue.įluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. Each lot of Kaleidoscope and SDS-PAGE prestained natural protein standards is individually calibrated for estimating the MW of sample proteins. Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range Prestained Nautral Standards for SDS-PAGE and western blotting provide a quick and easy way to monitor protein separation during electrophoresis and to assess transfer efficiency on blots.Molecular weight sizing of proteins after blot development Following SDS-PAGE, transfer proteins onto blotting membrane according to the manufacturer’s instructions.List Price: Your Price: Log in to see your price. 1.25 ml (5 x 250 µl), 10 pre-stained, Strep -tagged recombinant proteins (10250 kD), including three pink reference bands (25, 50, 75 kD), 250 applications. Precision Plus Protein All Blue Standards are a mixture of ten blue-stained recombinant proteins (10250 kD), including three reference bands (25, 50, and 75 kD). Monitoring blot transfer efficiency with easy orientation via multicolor standards Precision Plus Protein WesternC Blotting Standards, 1.25 ml. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple.Product Contentsġ - Precision Plus Protein WesternC Standards, 250 μl ( 1610376)ġ - Precision Protein™ StrepTactin-HRP Conjugate, 125 μl ( 1610381) These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development. Each pixel in a blot image has an x and y coordinate, in addition to an. A digital image of a blot can be thought of as data in three dimensions. A western blot image is made up of pixels, which contain information about how much signal was collected at each location in the image. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. A protein band is a feature that appears in a western blot image.